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The karyoplot shows the DraI (yellow), SspI (cyan), and <t>HpaI</t> (orange) restriction sites distribution genome-wide on the human reference genome assembly hg38. RE, restriction enzymes.
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The karyoplot shows the DraI (yellow), SspI (cyan), and HpaI (orange) restriction sites distribution genome-wide on the human reference genome assembly hg38. RE, restriction enzymes.

Journal: bioRxiv

Article Title: PRISM-Seq: An Ultra-sensitive Sequencing Approach For Mapping Lentiviral Integration Sites

doi: 10.64898/2025.12.20.695659

Figure Lengend Snippet: The karyoplot shows the DraI (yellow), SspI (cyan), and HpaI (orange) restriction sites distribution genome-wide on the human reference genome assembly hg38. RE, restriction enzymes.

Article Snippet: Purified MDA products were digested using three blunt-end restriction enzymes - HpaI (NEB #R0105L), SspI (NEB #R3132L), and DraI (NEB #R0129L) - to fragment genomic DNA and facilitate subsequent ligation-based integration site analysis.

Techniques: Genome Wide